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BOR - Papers in Press, published online ahead of print July 7, 2004.
Biol Reprod 2004, 10.1095/biolreprod.104.030155
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biolreprod.104.030155v1
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Submitted March 29, 2004
Returned for revision April 13, 2004
Accepted June 29, 2004

Gamete Biology


Peroxiredoxin 6 Is Up-Regulated in Bovine Oocytes and Cumulus Cells During In Vitro Maturation: Role of Intercellular Communication

Gregory Leyens , Benjamin Verhaeghe , Marie Landtmeters , Joëlle Marchandise , Bernard Knoops , and Isabelle Donnay *

* To whom correspondence should be addressed. E-mail: donnay{at}vete.ucl.ac.be.

Abstract
Peroxiredoxins are peroxidases involved in antioxidant defence and intracellular signalling. Expression of transcripts coding for peroxiredoxin 6 (PRDX6) has been previously described to be up-regulated in oocytes after in vitro maturation, a period during which general transcription decreases dramatically in oocytes. The aim of the present work was to evaluate PRDX6 regulation in bovine cumulus-oocyte complexes in relation to maturation and intercellular communication. PRDX6 expression was analysed by RT-PCR and Western blotting in oocytes and cumulus cells before and after in vitro maturation. PRDX6 was found to be up-regulated at the mRNA and protein levels in both cell types after maturation. The effect of paracrine and gap junctional communication on PRDX6 expression was then assessed by culturing cumulus clusters in presence or absence of denuded oocytes. While PRDX6 up-regulation in oocytes required intact cumulus-oocyte junctions, the presence of denuded oocytes was necessary but sufficient for the up-regulation to occur in cumulus cells. Finally, the influence of recombinant mouse growth differentiation factor-9 (GDF-9) on PRDX6 expression in cumulus cells was studied. GDF-9 induced cumulus expansion and PRDX6 up-regulation in bovine cumulus clusters. Altogether, our data suggest that PRDX6 up-regulation in cumulus-oocyte complexes during in vitro maturation is mutually regulated by both cell types: PRDX6 up-regulation in oocytes would require gap junctions with cumulus cells, while up-regulation in cumulus would depend on secretion of oocyte paracrine factor(s) with GDF-9 being a likely candidate.

Key words: Cumulus cells • Gene regulation • Oocyte development


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S. Mazerbourg and A. J.W. Hsueh
Genomic analyses facilitate identification of receptors and signalling pathways for growth differentiation factor 9 and related orphan bone morphogenetic protein/growth differentiation factor ligands
Hum. Reprod. Update, July 1, 2006; 12(4): 373 - 383.
[Abstract] [Full Text] [PDF]


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