Biol Reprod
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print May 19, 2004.
Biol Reprod 2004, 10.1095/biolreprod.104.030247
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
71/3/933    most recent
biolreprod.104.030247v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Han, S.
Right arrow Articles by DeJong, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Han, S.
Right arrow Articles by DeJong, J.
Agricola
Right arrow Articles by Han, S.
Right arrow Articles by DeJong, J.
Submitted March 29, 2004
Returned for revision April 14, 2004
Accepted May 7, 2004

Gamete Biology


A Short Core Promoter Drives Expression of the ALF Transcription Factor in Reproductive Tissues of Male and Female Mice

SangYoon Han , Wensheng Xie , Sok Ho Kim , Limin Yue , and Jeff DeJong *

* To whom correspondence should be addressed. E-mail: dejong{at}utdallas.edu.

Abstract
The control of gene expression in reproductive tissues involves a number of unique germ cell-specific transcription factors. One such factor, ALF (TFII{tau}), encodes a protein similar to the large subunit of general transcription factor TFIIA. To understand how this factor is regulated, we characterized transgenic mice that contain the ALF promoter linked to either {beta}-galactosidase or GFP reporters. The results show that as little as 133 base pairs are sufficient to drive developmentally-accurate and cell-specific expression. Transgene DNA was methylated and inactive in liver but could be reactivated in vivo by systemic administration of 5-aza, 2'-deoxycytidine. Fluorescence activated cell sorting allowed the identification of male germ cells that express the GFP transgene and provides a potential method to collect cells that might be under the control of a non-somatic transcription system. Finally, we find that transcripts from the endogenous ALF gene and derived transgenes can also be detected in whole ovary and in GV-stage oocytes of female mice. The ALF sequence falls into a class of germ cell promoters whose features include small size, high GC content, numerous CpG dinucleotides, and an apparent TATA-like element. Overall, the results define a unique core promoter that is active in both male and female reproductive tissues and suggest mouse ALF may have a regulatory role in male and female gametogenic gene expression programs.

Key words: Gamete Biology • Gametogenesis • Gene regulation


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
K. Tokuhiro, Y. Miyagawa, S. Yamada, M. Hirose, H. Ohta, Y. Nishimune, and H. Tanaka
The 193-Base Pair Gsg2 (Haspin) Promoter Region Regulates Germ Cell-Specific Expression Bidirectionally and Synchronously
Biol Reprod, March 1, 2007; 76(3): 407 - 414.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Kim, D. Li, Y. Cui, K. Mueller, W. C. Chears, and J. DeJong
Regulatory Factor Interactions and Somatic Silencing of the Germ Cell-specific ALF Gene
J. Biol. Chem., November 10, 2006; 281(45): 34288 - 34298.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
K. Kitamura, H. Nishimura, Y. Nishimune, and H. Tanaka
Identification of Human HAPRIN Potentially Involved in the Acrosome Reaction
J Androl, July 1, 2005; 26(4): 511 - 518.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2004 by the Society for the Study of Reproduction.