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Abstract
Ectopic testicular xenografting can be used to investigate
spermatogenesis and as an alternative means for generating
transgenic spermatozoa in many species. Improving the
efficiency of spermatogenesis in xenografted testicular
tissue will aid in the application of using this approach.
The present study was conducted to evaluate age related
differences in the establishment of spermatogenesis in
grafted testicular tissue from bulls between 2 and 16wk of
life. Testicular tissue was ectopically xenografted under
the skin on the backs of castrated nude mice and
subsequently evaluated for growth, testosterone production
and establishment of spermatogenesis 24wk after grafting.
The greatest weight increases occurred in donor tissue
from calves of the ages 2, 4, and 8wk compared to the ages
of 12 and 16wk. Recipient mouse serum testosterone
concentration was at normal physiological levels 24wk
after grafting and no significant differences were
detected between recipients grafted with testicular tissue
from bull calves of different ages. The development of
germ cells to elongated spermatids were observed in
seminiferous tubules of grafts from donor calves of the
ages 4, 8, 12, and 16wk but not observed in grafts from 2
wk donors which contained round spermatids as the most
advanced germ cell stage. Grafts from 8wk donors contained
a significantly higher (10-fold) average percentage of
seminiferous tubules with elongated spermatids than all
other donor ages. These data demonstrate differences in
the ability of testicular tissue from donor animals of
different ages to establish spermatogenesis following
ectopic testicular xenografting.
Key words:
Gamete Biology •
Testis •
Spermatogenesis
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