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Abstract
One method to identify the factors that control ovarian
function is to characterize the genes that are expressed
in ovary. In the present study, cDNA libraries from
fetal, neonatal, and prepubertal porcine ovaries,
pubertal ovaries on different days of the estrous cycle
(days 0 [follicle], 5, and 12 [follicle and corpus
luteum]), and follicles isolated from weaned sows (2, 4,
6, and 8 mm diameter) were constructed and sequenced. A
total of 22,176 cDNA were sequenced of which 15,613 were
of sufficient quality for clustering. Clustering of cDNA
resulted in 8,507 contigs; 6,294 (74%) of which were
comprised of a single sequence. Sixty-eight percent of
the contigs had consensus sequences that were homologous
to existing Tentative Consensus sequences (TC) or mature
transcripts (ET) in the TIGR Porcine Gene Index. The
consensus sequences were classified according to the Gene
Ontology Index. Most cDNA encoded proteins that were
components of the nucleus, ribosome, or mitochondrion.
The proteins primarily functioned in binding, catalysis,
and transport. Nearly 75% of the proteins were involved
in metabolism and cell growth and/or maintenance.
Analysis of the cDNA frequency across different libraries
demonstrated differential gene expression within
different size follicles, between follicles and corpora
lutea, and across developmental time-points. The
expression of selected genes (analyzed by ribonuclease
protection assay and northern blotting) was consistent
with the frequency of their respective cDNA in the
individual libraries. This porcine ovary unigene set will
be useful for identifying factors and mechanisms
controlling ovarian follicular development in a variety
of species.
Key words:
Ovary •
Corpus luteum •
Follicle •
Gene regulation
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