Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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BOR - Papers in Press, published online ahead of print October 27, 2004.
Biol Reprod 2004, 10.1095/biolreprod.104.032623
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Submitted June 2, 2004
Returned for revision June 28, 2004
Accepted September 30, 2004

Gamete Biology


Identification of A New, Testis-Specific Sperm Antigen Localized on the Principal Piece of the Spermatozoa Tail in the Fox (Vulpes vulpes)

Yann Verdier , Guillaume Farré , Nelly Rouet , Zoltan Kele , Tamás Janáky , and Franck Boué *

* To whom correspondence should be addressed. E-mail: f.boue{at}nancy.afssa.fr.

Abstract
Identification of fox (Vulpes vulpes) sperm antigens was carried out in order to assess their interest as potential target for a contraceptive vaccine. We report here the cloning and sequencing of fSP13, a fox sperm protein of 97 kDa. fSP13 was both auto- and iso-antigenic in foxes, as it was recognized by sera of foxes immunized with fox sperm proteins and vasectomised foxes. The NH2-terminal sequence of fSP13 was determined, and a piece of cDNA was amplified by RT PCR from testicular RNA. This piece was used to screen a cDNA library from fox testis by Southern blot. A sequence of 1662 bp was obtained, including a major open reading frame coding for 498 amino acid. Mass spectrometry analysis confirmed the position of the open reading frame, and the presence of post-transcriptional modifications. Analysis of the predicted amino acid sequence revealed no apparent transmembrane regions. Comparison of the protein sequence with the Prosite database demonstrated the presence of four potential N-linked glycosylation sites. fSP13 bears the closest amino acid similarity to two human sperm proteins: fibrousheathin 2 and testis-specific Calcium Binding Protein 86-VII. The deduced 80 N-terminal amino acid sequence also presents similarity with the RII{alpha} domain. By using a serum against fSP13, this antigen was localized on the principal piece of the fox spermatozoa. Northern blot analysis showed that fSP13 is specifically expressed in testis. fSP13 is one of the first fox sperm antigens to be cloned and sequenced.

Key words: Testis • Sperm


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M. S. Wakle, S. A. Joshi, and V. V. Khole
Monoclonal Antibody From Vasectomized Mouse Identifies a Conserved Testis-Specific Antigen TSA70
J Androl, November 1, 2005; 26(6): 761 - 771.
[Abstract] [Full Text] [PDF]


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