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Abstract
Microscopy of sectioned neonatal mouse ovaries established
the predominance of primordial follicles in day 3 samples
and the predominance of primary follicles by day 8. To
identify genetic determinants of the primordial to primary
follicle transition, the transcriptome of day 1 or day 3
mouse ovaries was contrasted by differential display with
that of day 8 ovaries. This manuscript examines one of
the upregulated genes, the novel gc-lrr (germ cell
leucine-rich repeat) gene whose transcript displayed 18-
and 127-fold increments from day 1 to days 3 and 8,
respectively. First noted by in situ in oocytes encased
by primary follicles, gc-lrr transcripts were continuously
expressed through the pre-ovulatory stage. The
transcripts declined upon the resumption of meiotic
maturation and were markedly diminished by the 2-cell
embryo. The corresponding 3,281bp full-length cDNA coded
for a 993 residue/104.6kDa germ cell specific protein. A
member of the multifunctional NACHT NTPase family, the
GC-LRR protein featured 14 iterations of the LRR domain, a
region implicated in protein-protein interaction. Protein
BLAST analysis of the GC-LRR sequence revealed 2
previously reported germ cell-specific homologs i.e. MATER
(Maternal Antigen That Embryos Require) and Rnh2
(RiboNuclease/Angiogenin Inhibitor 2). The structural
attributes, expression pattern, as well as the cellular
localization of MATER and Rnh2, largely conformed to those
reported for GC-LRR.
Key words:
Ovary •
Developmental biology •
Meiosis
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