| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Abstract
The effects of FSH, LH, and EGF on the dynamics of nuclear
maturation and subsequent embryo development were examined
in pig oocytes cultured conventionally or after
preincubation with cycloheximide (CHX). In conventional
culture, FSH or EGF significantly increased the rate of
attainment of MII for both gilt (50.0 ± 4.2% or
54.8 ± 4.3%; control 5.8 ± 1.8 %,
P<0.001) and sow (87.6 ± 3.4% or 78.8
± 3.9%; control 7.8 ± 2.5%, P<0.001)
oocytes. Gilt oocytes treated with both FSH and EGF showed
an additive response (93.7 ± 2.1%). Treatment with
LH had no effect. Preincubation with CHX caused the
majority (84-100%) of both gilt and sow oocytes to undergo
GVBD. Compared to those treated with LH and/or EGF (both
>80%), fewer FSH-treated oocytes reached MI (43.8
± 5.3%, P<0.001) by 14 h and MII (48.4
±5.9%, P<0.001) by 24 h, although the majority
(71%) did mature to MII by 36 h after removal of CHX.
After IVF, higher proportions of both CHX-pretreated and
untreated, FSH-exposed oocytes cleaved (71.3 ±
2.9% and 75.3 ± 3.1%) compared with those not
treated with FSH (37.7 ± 3.0% and 43.0 ±
2.9%; P<0.001). Pretreatment with CHX significantly
increased blastocyst yield for both FSH-treated (32.8
± 2.0% and 10.3 ± 1.5%, p<0.001) and
-untreated (16.7 ± 1.5% and 9.4 ± 1.2%,
p<0.001) oocytes. Polyspermy rates were unaffected. In
conclusion, pig oocytes meiotically arrested by CHX prior
to maturation retain and improve their developmental
competence. FSH stimulates nuclear maturation but slows
meiotic progression.
Key words:
Follicle-stimulating hormone •
In vitro fertilization •
Luteinizing hormone •
Meiosis •
Oocyte development
This article has been cited by other articles:
|
|
 |
|
  J Ye, J Coleman, M G Hunter, J Craigon, K H S Campbell, and M R Luck Physiological temperature variants and culture media modify meiotic progression and developmental potential of pig oocytes in vitro Reproduction, May 1, 2007; 133(5): 877 - 886. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Silvestre, J. Alfonso, E. Garcia-Mengual, I. Salvador, C. C. Duque, and I. Molina Effect of recombinant human follicle-stimulating hormone and luteinizing hormone on in vitro maturation of porcine oocytes evaluated by the subsequent in vitro development of embryos obtained by in vitro fertilization, intracytoplasmic sperm injection, or parthenogenetic activation J Anim Sci, May 1, 2007; 85(5): 1156 - 1160. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. Spikings, J. Alderson, and J. C. St. John Regulated Mitochondrial DNA Replication During Oocyte Maturation Is Essential for Successful Porcine Embryonic Development Biol Reprod, February 1, 2007; 76(2): 327 - 335. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
