Biol Reprod Lalor Postdoctoral Fellowships -- Application Deadline January 15, 2009
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print December 15, 2004.
Biol Reprod 2004, 10.1095/biolreprod.104.034793
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
72/4/1037    most recent
biolreprod.104.034793v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tremblay, K.
Right arrow Articles by Sirard, M.-A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tremblay, K.
Right arrow Articles by Sirard, M.-A.
Agricola
Right arrow Articles by Tremblay, K.
Right arrow Articles by Sirard, M.-A.
Submitted July 28, 2004
Returned for revision August 29, 2004
Accepted November 29, 2004

Gamete Biology


Expression of Cyclin B1 Messenger RNA Isoforms and Initiation of Cytoplasmic Polyadenylation in the Bovine Oocyte

Karine Tremblay , Christian Vigneault , Serge McGraw , and Marc-André Sirard *

* To whom correspondence should be addressed. E-mail: marc-andre.sirard{at}crbr.ulaval.ca.

Abstract
Oocytes can synthesize and store maternal mRNA in an inactive translational state until the start of in vitro maturation. Cytoplasmic polyadenylation, driven by 3' UTR cis-acting cytoplasmic polyadenylation element (CPE), is associated with translational activation of cyclin B1 mRNA during maturation. The main aim of this study was to investigate if bovine oocyte cyclin B1 mRNA undergoes cytoplasmic polyadenylation/translation during in vitro maturation, like in other species. We have found that cyclin B1 mRNA is present in two isoforms, consisting of the same open reading frame but with different 3' UTR lengths. Only the longest isoform (cyclin B1L) has a putative CPE sequence and other regulatory sequences and its mRNA level decreases during early embryo development. The polyadenylation state of cyclin B1L during in vitro maturation was studied. Results demonstrated that cyclin B1L bears a relatively long poly(A) tail in GV stage oocytes which is further lengthened at 10 h of maturation, before metaphase I. Interestingly, cyclin B1L bears a short poly(A) tail when the ovaries and the oocytes are transported and manipulated on ice to stop the polyadenylation process. Cytoplasmic polyadenylation occurs most probably during ovary transport in warm saline when oocytes are still in their follicular environment. Our results also show a link between cytoplasmic polyadenylation of cyclin B1 and translation/appearance of cyclin B1 protein before in vitro maturation.

Key words: Gamete Biology • Gene regulation • Meiosis • Ovum • Ovum pick-up / transport


This article has been cited by other articles:


Home page
 ReproductionHome page
A Bonnet, R Dalbies-Tran, and M A Sirard
Opportunities and challenges in applying genomics to the study of oogenesis and folliculogenesis in farm animals
Reproduction, February 1, 2008; 135(2): 119 - 128.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
H. Torner, N. Ghanem, C. Ambros, M. Holker, W. Tomek, C. Phatsara, H. Alm, M.-A. Sirard, W. Kanitz, K. Schellander, et al.
Molecular and subcellular characterisation of oocytes screened for their developmental competence based on glucose-6-phosphate dehydrogenase activity
Reproduction, February 1, 2008; 135(2): 197 - 212.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
S. Uzbekova, Y. Arlot-Bonnemains, J. Dupont, R. Dalbies-Tran, P. Papillier, S. Pennetier, A. Thelie, C. Perreau, P. Mermillod, C. Prigent, et al.
Spatio-Temporal Expression Patterns of Aurora Kinases A, B, and C and Cytoplasmic Polyadenylation-Element-Binding Protein in Bovine Oocytes During Meiotic Maturation
Biol Reprod, February 1, 2008; 78(2): 218 - 233.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2004 by the Society for the Study of Reproduction.