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Abstract
Bovine follicular atresia is associated with the apoptosis
of granulosa cells and the subsequent loss of oocyte
competence through the reduction of cellular contact such
as gap junctions. Several components of the IGF system are
thought to affect follicular atresia. While the IGFBPs are
present in varying quantities throughout follicular
development, IGFBP-5 appears to be present only during
atresia, in parallel with its regulation in other tissue
remodelling systems. However, no connection has yet been
made between atresia, low molecular weight IGFBP content
and oocyte quality in the bovine ovary. Caspases are
actively involved in ovarian follicular atresia, and
apoptosis in antral follicles is caspase-3 dependent.
Hence the aim was to investigate the use of these factors
in the assessment of oocyte quality and developmental
potential. Oocytes were aspirated, morphologically
classified and individually matured in vitro. The
follicular fluid and granulosa cells of these follicles
were analysed for IGFBP profile and caspase-3 activity
respectively. There was a significant correlation between
the presence of low molecular weight IGFBPs in bovine
follicular fluid and caspase-3 activity of granulosa cells
isolated from individual follicles. The highest percentage
of development to the blastocyst stage was observed in
oocytes from slightly atretic follicles. This group of
oocytes contained an equal proportion of grade 1-3
oocytes. These data demonstrate that low molecular weight
IGFBP profile is a more reliable method than the
traditional morphological assessment of oocytes and can be
used as an effective marker of developmentally competent
oocytes. Importantly, these results have implications for
the use of non-invasive follicular fluid markers for the
selection of competent oocytes to improve IVF outcomes.
Key words:
Ovary •
Apoptosis •
Follicle •
Oocyte development •
Ovum
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