Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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BOR - Papers in Press, published online ahead of print March 2, 2005.
Biol Reprod 2005, 10.1095/biolreprod.104.037036
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Submitted October 8, 2004
Returned for revision October 26, 2004
Accepted February 21, 2005

Female Reproductive Tract


Expression and Contribution of Three Different Isoforms of Prostaglandin E Synthase in the Bovine Endometrium

Julie Parent and Michel A. Fortier *

* To whom correspondence should be addressed. E-mail: mafortier{at}crchul.ulaval.ca.

Abstract
Prostaglandins are involved in the regulation of several reproductive processes such as ovulation, luteolysis and establishment of pregnancy. PGE2 appears to favor establishment of pregnancy in most mammals studied so far. The primary enzymes involved in the production of PGE2 from arachidonic acid are cyclooxygenases and prostaglandin E synthases. Three PGES have been identified in human but in the bovine, microsomal PGES2 and cytosolic PGES genes have neither been cloned nor associated to any physiological processes. The present study was undertaken to clone bovine MPGES2 and CPGES and to report on their regulation in the endometrium during the estrous cycle. CPGES mRNA expression declines progressively during the cycle; its protein is not modulated according to a precise pattern. MPGES2 mRNA and protein expression decrease from the beginning of the cycle until days 13-15 and then increase until ovulation. Immunohistochemichal analysis reveals that both enzymes are located in luminal epithelial and glandular epithelial cells, and at a lower level in stromal cells. In addition, using the bovine endometrial cell line BEND where higher accumulation of PGE2 is observed following treatment with PMA and TNF-{alpha}, we have found an associated increase of MPGES1 and COX2 but not CPGES or MPGES2 protein expression. Together, our results suggest that MPGES1 is not the only PGES present in the bovine endometrium, but is the main enzyme associated with increased PGE2 production in vitro.

Key words: Female Reproductive Tract • Mechanisms of Hormone Action • Pregnancy • Ovulatory cycle • Uterus


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