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BOR - Papers in Press, published online ahead of print May 18, 2005.
Biol Reprod 2005, 10.1095/biolreprod.104.039180
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Submitted December 16, 2004
Returned for revision January 20, 2005
Accepted May 13, 2005

Testis


Identification of a New Variant of PDE1A Calmodulin-Stimulated Cyclic Nucleotide Phosphodiesterase Expressed in Mouse Sperm

Valeria Vasta , William K. Sonnenburg , Chen Yan , Scott H. Soderling , Masami Shimizu-Albergine , and Joseph A. Beavo *

* To whom correspondence should be addressed. E-mail: beavo{at}u.washington.edu.

Abstract
In mature sperm, cAMP plays an important role as a second messenger regulating functions that include capacitation, the acrosome reaction, motility, and in some cases, chemosensing. We have cloned from mouse testis a novel calmodulin-stimulated cyclic nucleotide phosphodiesterase 1A isoform, Pde1a_v7. (mmPDE1A7) which arises from an alternative transcription start in the cyclic nucleotide phosphodiesterase 1A gene. The open reading frame is predicted to encode a polypeptide with a molecular mass of 52 kDa. Two further variants of this form which contain two additional new exons arise from alternative splicing. Analysis of testis cDNA by real time PCR indicates that the Pde1A_v7 transcript variant is the most abundant. The PDE1A_v7 protein uniquely lacks the first amino-terminal calmodulin-binding domain, but does possess an inhibitory domain and a second calmodulin-binding site shared with other variants. In vitro translation of the corresponding Pde1a_v7 cDNA produced a 52 kDa polypeptide having cyclic nucleotide hydrolytic activity, which was stimulated three-fold by calcium-bound calmodulin. Immunoprecipitation of cyclic nucleotide phosphodiesterase 1 activity from detergent extracts of mouse sperm revealed a major protein of the size expected for PDE1A_v7 and the Immunocytochemical staining for cyclic nucleotide phosphodiesterase 1A in mouse sperm showed intense immunoreactivity in the tail only. These observations along with the PCR data strongly suggest that this new variant PDE1A_v7 is the major form of cyclic nucleotide phosphodiesterase 1A expressed in mature sperm and therefore likely to play an important role in cyclic nucleotide regulation of mature sperm function.

Key words: Calcium • Signal transduction • Sperm capacitation • Sperm maturation • Sperm motility and transport


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A. T. Bender and J. A. Beavo
Cyclic Nucleotide Phosphodiesterases: Molecular Regulation to Clinical Use
Pharmacol. Rev., September 1, 2006; 58(3): 488 - 520.
[Abstract] [Full Text] [PDF]


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