Submitted January 28, 2005
Returned for revision March 2, 2005
Accepted May 24, 2005
Pregnancy
Adrenomedullin Enhances Invasion by Trophoblast Cell Lines
Xiaoquan Zhang ,
Kortney E. Green ,
Chandra Yallampalli ,
and
Yuan Lin Dong *
* To whom correspondence should be addressed. E-mail: ydong{at}utmb.edu.
Abstract
We have tested the hypothesis that adrenomedullin (ADM), a
multi-functional peptide hormone, works as a trophoblast pro-invasion factor. Our results showed that ADM
receptor components- calcitonin receptor-like receptor
(CALCRL) and receptor activity modifying proteins (RAMPs) mRNA and proteins were expressed by human
choriocarcinoma JAr cells and first-trimester
cytotrophoblast HTR-8/SV neo cells. ADM stimulates both JAr and HTR-8/SV neo cell proliferation. The invasion capabilities of JAr cells and HTR-8/SV neo cells were also enhanced by ADM, and this was associated with increased gelatinolytic activity and reduced plasminogen activator inhibitor-1 mRNA expression (SERPINE1). Our data supports the notion that ADM may be involved in the human implantation process via regulating trophoblast proliferation and differentiation.
Key words:
Pregnancy •
Growth factors •
Implantation •
Placenta •
Trophoblast
