| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Abstract
Mammalian sperm acquire fertilization capacity after
residing in the female tract in a process known as
capacitation. This study examined whether cholesterol
efflux during capacitation alters the biophysical
properties of the sperm plasma membrane by potentially
reducing the extent of lipid raft domains as analyzed by
the isolation of detergent-resistant membrane fractions
using sucrose gradients. In addition, this work
investigated whether dissociation of the detergent
resistant membrane fraction during capacitation alters
resident sperm raft proteins. Mouse sperm proteins
associated with such fractions were studied by silver
staining, tandem mass spectrometry and Western blotting.
Caveolin 1 was identified in sperm lipid rafts in
multimeric states, including a high molecular weight
oligomer sensitive to degradation under reducing
conditions at high pH. Capacitation resulted in reduction
of the light buoyant density detergent-resistant membrane
fraction and decreased the array of proteins isolated
within this fraction including loss of the high molecular
weight caveolin 1 oligomers. Proteomic analysis of sperm
proteins isolated in the light buoyant density fraction
identified several proteins, including Hexokinase 1,
testis serine protease 1 and 2, TEX101, Hyaluronidase
(PH20, SPAM1), Facilitated glucose transporter 3, Lactate
Dehydrogenase A, Carbonic Anhydrase IV, IZUMO,
Pantophysin, Basigin and CRISP1. Capacitation also
resulted in a significant reduction in sperm labeling by
the fluorescent lipid analogue DiIC16 indicating
capacitation alters the liquid-ordered domains in the
sperm plasma membrane. The observations that capacitation
alters the protein composition of the detergent-resistant
membrane fractions is consistent with the hypothesis that
cholesterol efflux during capacitation dissociates lipid
raft constituents initiating signaling events leading to
sperm capacitation.
Key words:
Testis •
Fertilization •
Signal transduction •
Sperm •
Sperm capacitation
This article has been cited by other articles:
|
|
 |
|
  F. Odet, C. Duan, W. D Willis, E. H Goulding, A. Kung, E. M Eddy, and E. Goldberg Expression of the Gene for Mouse Lactate Dehydrogenase C (Ldhc) Is Required for Male Fertility Biol Reprod, July 1, 2008; 79(1): 26 - 34. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Girouard, G. Frenette, and R. Sullivan Seminal Plasma Proteins Regulate the Association of Lipids and Proteins Within Detergent-Resistant Membrane Domains of Bovine Spermatozoa Biol Reprod, May 1, 2008; 78(5): 921 - 931. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. S Griffiths, K. A Miller, D. S Galileo, and P. A Martin-DeLeon Murine SPAM1 is secreted by the estrous uterus and oviduct in a form that can bind to sperm during capacitation: acquisition enhances hyaluronic acid-binding ability and cumulus dispersal efficiency Reproduction, March 1, 2008; 135(3): 293 - 301. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. van Gestel, I. A. Brewis, P. R. Ashton, J. F. Brouwers, and B. M. Gadella Multiple proteins present in purified porcine sperm apical plasma membranes interact with the zona pellucida of the oocyte Mol. Hum. Reprod., July 1, 2007; 13(7): 445 - 454. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Selvaraj, D. E. Buttke, A. Asano, J. L. Mcelwee, C. A. Wolff, J. L. Nelson, A. V. Klaus, G. R. Hunnicutt, and A. J. Travis GM1 Dynamics as a Marker for Membrane Changes Associated With the Process of Capacitation in Murine and Bovine Spermatozoa J Androl, July 1, 2007; 28(4): 588 - 599. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. E. Furland, G. M. Oresti, S. S. Antollini, A. Venturino, E. N. Maldonado, and M. I. Aveldano Very Long-chain Polyunsaturated Fatty Acids Are the Major Acyl Groups of Sphingomyelins and Ceramides in the Head of Mammalian Spermatozoa J. Biol. Chem., June 22, 2007; 282(25): 18151 - 18161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.N. Jha, A.M. Salicioni, E. Arcelay, O. Chertihin, S. Kumari, J.C. Herr, and P.E. Visconti Evidence for the involvement of proline-directed serine/threonine phosphorylation in sperm capacitation Mol. Hum. Reprod., December 1, 2006; 12(12): 781 - 789. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-H. Huang, C.-C. Wei, Y.-H. Su, B.-T. Wu, Y.-Y. Ciou, C.-F. Tu, T. G. Cooper, C.-H. Yeung, S.-T. Chu, M.-T. Tsai, et al. Localization and Characterization of an Orphan Receptor, Guanylyl Cyclase-G, in Mouse Testis and Sperm Endocrinology, October 1, 2006; 147(10): 4792 - 4800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Krisfalusi, K. Miki, P. L. Magyar, and D. A. O'Brien Multiple Glycolytic Enzymes Are Tightly Bound to the Fibrous Sheath of Mouse Spermatozoa Biol Reprod, August 1, 2006; 75(2): 270 - 278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Chen, G. Griffiths, D. S. Galileo, and P. A. Martin-DeLeon Epididymal SPAM1 Is a Marker for Sperm Maturation in the Mouse Biol Reprod, May 1, 2006; 74(5): 923 - 930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Buttke, J. L. Nelson, P. N. Schlegel, G. R. Hunnicutt, and A. J. Travis Visualization of GM1 with Cholera Toxin B in Live Epididymal Versus Ejaculated Bull, Mouse, and Human Spermatozoa Biol Reprod, May 1, 2006; 74(5): 889 - 895. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Nolan, L. Wu, H. J. Bang, S. A. Jelinsky, K. P. Roberts, T. T. Turner, G. S. Kopf, and D. S. Johnston Identification of Rat Cysteine-Rich Secretory Protein 4 (Crisp4) as the Ortholog to Human CRISP1 and Mouse Crisp4 Biol Reprod, May 1, 2006; 74(5): 984 - 991. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Gibbs, M. J. Scanlon, J. Swarbrick, S. Curtis, E. Gallant, A. F. Dulhunty, and M. K. O'Bryan The Cysteine-rich Secretory Protein Domain of Tpx-1 Is Related to Ion Channel Toxins and Regulates Ryanodine Receptor Ca2+ Signaling J. Biol. Chem., February 17, 2006; 281(7): 4156 - 4163. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
