Biol Reprod Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print July 6, 2005.
Biol Reprod 2005, 10.1095/biolreprod.105.043117
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
73/5/967    most recent
biolreprod.105.043117v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kezele, P.
Right arrow Articles by Skinner, M. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kezele, P.
Right arrow Articles by Skinner, M. K.
Agricola
Right arrow Articles by Kezele, P.
Right arrow Articles by Skinner, M. K.
Submitted April 21, 2005
Returned for revision May 6, 2005
Accepted July 5, 2005

Ovary


Keratinocyte Growth Factor Acts as a Mesenchymal Factor that Promotes Ovarian Primordial to Primary Follicle Transition

Phillip Kezele , Eric E. Nilsson , and Michael K. Skinner *

* To whom correspondence should be addressed. E-mail: skinner{at}mail.wsu.edu.

Abstract
An important but poorly understood process in ovarian biology is the transition of the developmentally arrested primordial follicle to the developing primary follicle. Interactions between the epithelial and mesenchymal cells of the follicle are critical for the coordination of ovarian follicle development. The mesenchymal growth factor keratinocyte growth factor (KGF) (i.e. fibroblast growth factor-7, FGF7) and the epithelial growth factor Kit Ligand (KITL) are known to interact to coordinate the growth of later stage antral follicles. The hypothesis tested in the current study is that KGF acts as a mesenchymal factor to promote the primordial to primary follicle transition. A postnatal 4-day-old rat ovary organ culture system was used to investigate the actions of KGF. KGF treatment promoted 65% of follicles to undergo the primordial to primary follicle transition, while only 45% undergo development in control ovaries. Neutralizing antibody for KGF was found to attenuate the stimulatory action of KITL, but neutralizing antibody for KITL was not able to attenuate the stimulatory action of KGF. Further analysis demonstrated that KGF was found to stimulate the expression of KITL (i.e. mRNA levels) by granulosa cells. KITL in turn was found to stimulate the expression of KGF to create a positive feedback loop. Interestingly, KGF expression was localized to selected mesenchymal cells (i.e. precursor theca cells) surrounding the developing primordial follicle. Observations suggest developing granulosa cells of the primordial follicles produce KITL that helps recruit precursor theca cells to the follicle that then produce KGF that acts on the granulosa to amplify KITL expression and support primordial follicle development. KGF appears to be a mesenchymal factor that promotes the primordial to primary follicle transitions.

Key words: Ovary • Follicle • Granulosa cells • Oocyte development • Theca cells


This article has been cited by other articles:


Home page
 ReproductionHome page
G. Dole, E. E Nilsson, and M. K Skinner
Glial-derived neurotrophic factor promotes ovarian primordial follicle development and cell-cell interactions during folliculogenesis
Reproduction, May 1, 2008; 135(5): 671 - 682.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J Buratini Jr., M.G.L Pinto, A.C Castilho, R.L Amorim, I.C Giometti, V.M Portela, E.S Nicola, and C.A Price
Expression and Function of Fibroblast Growth Factor 10 and Its Receptor, Fibroblast Growth Factor Receptor 2B, in Bovine Follicles
Biol Reprod, October 1, 2007; 77(4): 743 - 750.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
E. Nilsson, N. Rogers, and M. K Skinner
Actions of anti-Mullerian hormone on the ovarian transcriptome to inhibit primordial to primary follicle transition
Reproduction, August 1, 2007; 134(2): 209 - 221.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
Y. Choi, Y. Qin, M. F. Berger, D. J. Ballow, M. L. Bulyk, and A. Rajkovic
Microarray Analyses of Newborn Mouse Ovaries Lacking Nobox
Biol Reprod, August 1, 2007; 77(2): 312 - 319.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
Y. Wang, J. Li, C. Ying Wang, A. H. Yan Kwok, and F. C. Leung
Epidermal Growth Factor (EGF) Receptor Ligands in the Chicken Ovary: I. Evidence for Heparin-Binding EGF-Like Growth Factor (HB-EGF) as a Potential Oocyte-Derived Signal to Control Granulosa Cell Proliferation and HB-EGF and Kit Ligand Expression
Endocrinology, July 1, 2007; 148(7): 3426 - 3440.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
P. G Knight and C. Glister
TGF-{beta} superfamily members and ovarian follicle development.
Reproduction, August 1, 2006; 132(2): 191 - 206.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
E. E Nilsson, C. Detzel, and M. K Skinner
Platelet-derived growth factor modulates the primordial to primary follicle transition.
Reproduction, June 1, 2006; 131(6): 1007 - 1015.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
K.J. Hutt, E.A. McLaughlin, and M.K. Holland
Kit ligand and c-Kit have diverse roles during mammalian oogenesis and folliculogenesis
Mol. Hum. Reprod., February 1, 2006; 12(2): 61 - 69.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2005 by the Society for the Study of Reproduction.