Gamete Biology

Age-Associated Changes in Mouse Oocytes During Post-Ovulatory In Vitro Culture: Possible Role for Meiotic Kinases and Survival Factor BCL2

Abstract
In order to elucidate molecular mechanisms underlying oocyte senescence, we investigated whether oocytes from female mice of advanced reproductive age exhibit a precocious post-ovulatory aging that, in turn, may be responsible for the precocious activation of an apoptotic program. During a 9-hr in vitro culture the frequency of oocytes showing MII aberrations, spontaneous activation and cellular fragmentation increased in old oocytes (P<0.05), whereas it did not change in the young group. In old oocytes the activities of MPF (a complex of the cyclin-dependent kinase cdc2 and cyclin B1) and MAPK (mitogen-activated protein kinase) decreased precociously showing a first drop as early as 3 hr after the beginning of in vitro culture (P<0.05). Immunoblotting and immunocytochemical analysis revealed that in oocytes of the old group reduction of BCL2 expression at protein level occurred earlier than in the young group (P<0.05) and was not associated to the loss of BCL2 transcripts detected by RT-PCR. These changes are followed by an abrupt increase of the rate of TUNEL-positive oocytes after 24 hr of culture to a value of 67±6%. Exposure of young oocytes to 20 µM roscovitine or 20 µM U0126, specific inhibitors of MPF and MAPK, resulted in the decreased percentage of oocytes showing positive immunostaining for BCL2 and in an increased rate of DNA fragmentation. Present results suggest that the developmental competence of oocytes ovulated by aging mice may be negatively influenced by a down-regulation of MPF and MAPK activities that in turn induces the activation of a pro-apoptotic signalling pathway.

Key words: Aging • Apoptosis • Fertilization • Kinases • Oocyte development

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