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BOR - Papers in Press, published online ahead of print April 26, 2006.
Biol Reprod 2006, 10.1095/biolreprod.106.051136
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biolreprod.106.051136v1
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Submitted January 23, 2006
Returned for revision March 1, 2006
Accepted April 20, 2006

Testis


PSPC1, NONO, and SFPQ Are Expressed in Mouse Sertoli Cells and May Function as Coregulators of Androgen Receptor-Mediated Transcription

Sho Kuwahara , Asako Ikei , Yusuke Taguchi , Yoshiaki Tabuchi , Nariaki Fujimoto , Masuo Obinata , Seiichi Uesugi , and Yasuyuki Kurihara *

* To whom correspondence should be addressed. E-mail: kurihara{at}ynu.ac.jp.

Abstract
In Sertoli cells of testis, androgen receptor-regulated gene transcription plays indispensable roles in maintaining spermatogenesis. Androgen receptor activity is modulated by a number of coregulators which are associated with androgen receptor. Non-POU-domain-containing, octamer binding protein (NONO), a member of DBHS-containing proteins, complexes with androgen receptor and functions as a coactivator for the receptor. Paraspeckle protein 1 alpha isoform (PSPC1, previously known as PSP1) and Splicing factor, proline- and glutamine-rich (SFPQ, previously known as PSF), other members of DBHS-containing proteins, are also found in androgen receptor complexes, suggesting that these DBHS-containing proteins may cooperatively regulate androgen receptor-mediated gene transcription. We demonstrated that PSPC1, NONO and SFPQ are coexpressed in Sertoli cell line TTE3 and interact reciprocally. Effect of the DBHS-containing proteins on the transcriptional activity was assessed using the construct containing androgen-responsive elements followed by a luciferase gene. The results showed that all the DBHS-containing proteins activate androgen receptor-mediated transcription, and PSPC1 is the most effective coactivator among them. Furthermore, we confirmed the presence of PSPC1, NONO and SFPQ proteins in Sertoli cells of adult mouse testis sections. These observations suggest that PSPC1, NONO and SFPQ form complexes with each other in Sertoli cells and may regulate androgen receptor-mediated transcriptional activity.

Key words: Testis • Androgen receptor • Gene regulation • Sertoli cells • Spermatogenesis


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Z. Zhang, J. Hill, M. Holland, Y. Kurihara, and K. L. Loveland
Bovine Sertoli Cells Colonize and Form Tubules in Murine Hosts Following Transplantation and Grafting Procedures
J Androl, July 1, 2008; 29(4): 418 - 430.
[Abstract] [Full Text] [PDF]


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