Biol Reprod
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print October 25, 2006.
Biol Reprod 2006, 10.1095/biolreprod.106.056226
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
76/2/250    most recent
biolreprod.106.056226v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yoon, M. S.
Right arrow Articles by Han, J.-S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoon, M. S.
Right arrow Articles by Han, J.-S.
Agricola
Right arrow Articles by Yoon, M. S.
Right arrow Articles by Han, J.-S.
Submitted August 7, 2006
Returned for revision August 28, 2006
Accepted October 23, 2006

Female Reproductive Tract


Phospholipase D1 as a Key Enzyme for Decidualization in Human Endometrial Stromal Cells

Mee Sup Yoon , Jun Bon Koo , Yong Geon Jeong , Yong Seok Kim , Jung Han Lee , Hyae Jin Yun , Ki Sung Lee , and Joong-Soo Han *

* To whom correspondence should be addressed. E-mail: jshan{at}hanyang.ac.kr.

Abstract
Using a primary cell culture system of human endometrial stromal cells (ES cells), we investigated the role of phospholipase D (PLD) in 8-Br-cAMP-induced decidualization, which is a morphological and biological differentiation process. When treated with 0.5 mM 8-Br-cAMP for 12 days, ES cells were transformed into a decidualized morphology and produced significant amounts of prolactin (PRL) and insulin-like growth factor binding protein 1 (IGFBP1). Simultaneously, the activity and expression level of PLD1 increased also. In addition, removal of 8-Br-cAMP from decidualized ES cells restored the undifferentiated state, accompanied with a decrease of PLD1 promoter activity and PLD1 expression. Overexpression of dominant negative (DN)-PLD1 inhibited the morphological changes induced by 0.5 mM 8-Br-cAMP, whereas overexpression of PLD1 induced morphological changes in the absence of 0.5 mM 8-Br-cAMP treatment. Moreover, knockdown of PLD1 by siRNA and blockage of PLD by 0.3% 1-butanol treatment decreased PRL/IGFBP1 mRNA expression, whereas overexpression of PLD1 increased PRL/IGFBP1 mRNA expression. Treatment of ES cells with phosphatidic acid (PA) for 3 days induced PRL mRNA expression and morphological change, implying that PA as an end product of PLD activation induced decidualization. In addition, pretreatment of ES cells with mepacrine decreased PRL/IGFBP1 expression and morphological change, whereas pretreatment with propranolol did not change, compared to cAMP-treated cells, suggesting that PA induces decidualization through phospholipase A2 (PLA2G1B). Taken together, these results suggest that PLD1 regulates 8-Br-cAMP-induced decidualization through PLA2G1B, and that upregulation of PLD1 is essential for decidualization of ES cells.

Key words: Developmental biology


This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
M.-S. Yoon and J. Chen
PLD regulates myoblast differentiation through the mTOR-IGF2 pathway
J. Cell Sci., February 1, 2008; 121(3): 282 - 289.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2006 by the Society for the Study of Reproduction.