Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

BOR - Papers in Press, published online ahead of print January 3, 2007.
Biol Reprod 2007, 10.1095/biolreprod.106.056770
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
76/4/701    most recent
biolreprod.106.056770v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Busso, D.
Right arrow Articles by Cuasnicu, P. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Busso, D.
Right arrow Articles by Cuasnicu, P. S.
Agricola
Right arrow Articles by Busso, D.
Right arrow Articles by Cuasnicu, P. S.
Submitted August 28, 2006
Returned for revision October 4, 2006
Accepted December 29, 2006

Gamete Biology


Evidence for the Involvement of Testicular Protein CRISP2 in Mouse Sperm-Egg Fusion

Dolores Busso , Nadia M. Goldweic , Masaru Hayashi , Masanori Kasahara , and Patricia S. Cuasnicu *

* To whom correspondence should be addressed. E-mail: cuasnicu{at}dna.uba.ar.

Abstract
Protein CRISP2, originally known as Tpx-1, is a cysteine-rich secretory protein specifically expressed in male haploid germ cells. Although likely to be involved in gamete interaction, evidence for a functional role of CRISP2 in fertilization still remains poor. In the present work we have used a mouse model to examine the subcellular localization of CRISP2 in sperm and its involvement in the different stages of fertilization. Results from both indirect immunofluorescence and protein extraction experiments indicate that mouse CRISP2 is an intra-acrosomal component that remains associated with sperm after capacitation and the acrosome reaction. In vitro fertilization assays using zona pellucida-intact mouse eggs show that an antibody against the protein significantly decreases the percentage of penetrated eggs with a coincident accumulation of perivitelline sperm. The failure to inhibit zona pellucida penetration excludes a detrimental effect of the antibody on sperm motility and/or acrosome reaction, thus supporting a specific participation of CRISP2 at the sperm-egg fusion step. In agreement with this evidence, recombinant mouse CRISP2 (recCRISP2) specifically binds to the fusogenic area of mouse eggs as previously reported for rat CRISP1, an epididymal protein involved in gamete fusion. In vitro competition studies show that incubation of mouse zona-free eggs with a fixed concentration of recCRISP2 and increasing amounts of rat CRISP1 reduces the binding of recCRISP2 to the egg, suggesting that both proteins interact with common egg complementary sites. These results indicate that testicular CRISP2, as epididymal CRISP1, would be involved in sperm-egg fusion through its binding to complementary sites on the egg surface, supporting the idea of a functional cooperation between homologous molecules to ensure the success of fertilization.

Key words: Testis • Fertilization • Ovum • Sperm


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
M. L. Vadnais, D. N. Foster, and K. P. Roberts
Molecular Cloning and Expression of the CRISP Family of Proteins in the Boar
Biol Reprod, December 1, 2008; 79(6): 1129 - 1134.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
D. Jamsai, A. Reilly, S.J. Smith, G.M. Gibbs, H.W.G. Baker, R.I. McLachlan, D.M. de Kretser, and M.K. O'Bryan
Polymorphisms in the human cysteine-rich secretory protein 2 (CRISP2) gene in Australian men
Hum. Reprod., September 1, 2008; 23(9): 2151 - 2159.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
R. Yamaguchi, K. Yamagata, H. Hasuwa, E. Inano, M. Ikawa, and M. Okabe
Cd52, known as a major maturation-associated sperm membrane antigen secreted from the epididymis, is not required for fertilization in the mouse.
Genes Cells, August 1, 2008; 13(8): 851 - 861.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
D. Jamsai, D. M Bianco, S. J Smith, D. J Merriner, J. D Ly-Huynh, A. Herlihy, B. Niranjan, G. M Gibbs, and M. K O'Bryan
Characterization of gametogenetin 1 (GGN1) and its potential role in male fertility through the interaction with the ion channel regulator, cysteine-rich secretory protein 2 (CRISP2) in the sperm tail
Reproduction, June 1, 2008; 135(6): 751 - 759.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
A. Chandra, K. R. Srinivasan, F. Jamal, P. K. Mehrotra, R. L. Singh, and A. Srivastav
Post-translational modifications in glycosylation status during epididymal passage and significance in fertility of a 33 kDa glycoprotein (MEF3) of rhesus monkey (Macaca mulatta)
Reproduction, June 1, 2008; 135(6): 761 - 770.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
D. Busso, D. J Cohen, J. A Maldera, A. Dematteis, and P. S Cuasnicu
A Novel Function for CRISP1 in Rodent Fertilization: Involvement in Sperm-Zona Pellucida Interaction
Biol Reprod, November 1, 2007; 77(5): 848 - 854.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
G. M. Gibbs, D. M. Bianco, D. Jamsai, A. Herlihy, S. Ristevski, R. J. Aitken, D. M. d. Kretser, and M. K. O'Bryan
Cysteine-Rich Secretory Protein 2 Binds to Mitogen-Activated Protein Kinase Kinase Kinase 11 in Mouse Sperm
Biol Reprod, July 1, 2007; 77(1): 108 - 114.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2007 by the Society for the Study of Reproduction.