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BOR - Papers in Press, published online ahead of print October 25, 2006.
Biol Reprod 2006, 10.1095/biolreprod.106.056895
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Submitted August 30, 2006
Returned for revision September 14, 2006
Accepted October 5, 2006

Reproductive Technology


Production of Functional Spermatids from Mouse Germline Stem Cells in Ectopically Reconstituted Seminiferous Tubules

Kaoru Kita , Takeshi Watanabe , Kimito Ohsaka , Hirofumi Hayashi , Yoshinobu Kubota , Yoji Nagashima , Ichiro Aoki , Hideki Taniguchi , Toshiaki Noce , Kimiko Inoue , Hiromi Miki , Narumi Ogonuki , Hiromitsu Tanaka , Atsuo Ogura , and Takehiko Ogawa *

* To whom correspondence should be addressed. E-mail: ogawa{at}med.yokohama-cu.ac.jp.

Abstract
Testicular germ cell transplantation into the seminiferous tubules is at present the only way to induce spermatogenesis from a given source of spermatogonial stem cells. Here we show an alternative method which harnesses the self-organizing ability of testicular somatic cells. The testicular cells of embryonic or neonatal mice or rats and of newborn pigs were dissociated into single cells. Each of them re-organized into a tubular structure following implantation into the subcutis of immunodeficient mice. When mouse germline stem (GS) cells, derived from spermatogonial stem cells and expanded in culture, were intermingled with testicular cells of rodents, they were integrated in the reconstituted tubules and differentiated beyond meiosis into spermatids. Normal offspring were produced by the microinjection of those spermatids into oocytes. This method could be applicable to various mammalian species and useful for producing functional gametes from GS cells in a xeno-ectopic environment.

Key words: Assisted Reproductive Technology • Testis • Gametogenesis • Meiosis • Spermatogenesis


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[Abstract] [Full Text] [PDF]


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