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BOR - Papers in Press, published online ahead of print December 13, 2006.
Biol Reprod 2006, 10.1095/biolreprod.106.058032
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Submitted October 10, 2006
Returned for revision October 27, 2006
Accepted December 12, 2006

Reproductive Technology


Heterotopic Transplantation of Testes in Newly Hatched Chickens and Subsequent Production of Offspring via Intramagnal Insemination

Yonghong Song and Frederick Silversides *

* To whom correspondence should be addressed. E-mail: silversidesf{at}agr.gc.ca.

Abstract
Transplantation of testicular tissue onto the back of immunodeficient nude mice provides a tool to examine testicular development and preserve fertility in mammals. There is no immunodeficient model in birds, but we recently transplanted ovarian tissue between newly hatched chicks from two lines of chickens and produced donor-derived offspring, showing that experimental transplantation is possible in newly hatched chicks. In the present study, testicular tissue from newly hatched Barred Plymouth Rock (BPR) chicks was transplanted under the skin of the back, under the skin of the abdomen, or in the abdomen of White Leghorn chicks that had been surgically castrated and immunocompromised. Recipient birds were sacrificed at ten months of age. Transplanted tissue was observed in one of five hosts receiving tissue under the skin of the back, two of five hosts receiving tissue under the skin of the abdomen and three of five chicks with grafts inside the abdominal cavity. In recipients with no regeneration of host testes, testicular transplants grew to the size of normal testes and histological analysis showed active spermatogenesis. Subsequent collection of sperm from two successful transplants and surgical insemination of the sperm into the magna of the oviducts of BPR hens resulted in the production of 24 donor-derived chicks. These results demonstrate that the combination of testicular tissue transplantation with intramagnal insemination can produce viable, normal chicks which could provide a simple approach for the recuperation of live offspring in avian species.

Key words: Assisted Reproductive Technology • Testis • Sperm • Spermatogenesis


This article has been cited by other articles:


Home page
 Poult. Sci.Home page
Y. Song and F. G. Silversides
Production of Offspring from Cryopreserved Chicken Testicular Tissue
Poult. Sci., July 1, 2007; 86(7): 1390 - 1396.
[Abstract] [Full Text] [PDF]


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