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BOR - Papers in Press, published online ahead of print February 21, 2007.
Biol Reprod 2007, 10.1095/biolreprod.106.058222
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Submitted October 18, 2006
Returned for revision November 14, 2006
Accepted February 21, 2007

Testis


Analysis of Gene Expression in Bovine Testis Tissue Prior to Ectopic Testis Tissue Xenografting and During the Grafting Period

Jonathan A. Schmidt , Jeanene M. de Avila , and Derek J. McLean *

* To whom correspondence should be addressed. E-mail: dmclean{at}wsu.edu.

Abstract
The purpose of this study was to identify factors that contribute to bovine testis development and donor age dependent differences in the ability of bovine ectopic testis tissue grafts to produce elongated spermatids. We used real-time RT-PCR and microarrays to evaluate and identify the expression of genes involved in Sertoli and germ cell development in bovine testis tissue. Testis tissue was obtained from 2, 4, and 8 week old bull calves and was immediately grafted. Grafted bovine testis tissue was removed from mice, RNA was isolated from the grafts and real-time RT-PCR was used to evaluate gene expression during the grafting period. Additionally, gene expression in donor tissue was analyzed using Affymetrix Bovine GeneChips to identify differentially expressed genes. Examination of gene expression in testis tissue grafts indicated that Sertoli cell specific genes were lower in 8 week donor tissue grafts compared to the other aged donors. Additionally, the expression of KIT, a germ cell specific gene was low in testis tissue grafts. Microarray analysis of donor tissue identified several genes that are involved in angiogenesis or tissue growth that were differentially expressed in 2, 4, and 8 week old bovine testes. Expression of these genes (angiogenin, transgelin, thrombomodulin, early growth response 1, insulin like growth factor 2, and insulin like growth factor binding protein 3) were lower in testis tissue from older animals. Using this data, it will be possible in the future to manipulate the testis xenograft microenvironment to improve the efficiency of sperm production within the graft.

Key words: Testis • Developmental biology • Gene regulation • Spermatogenesis


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C. Wyns, A. Van Langendonckt, F.-X. Wese, J. Donnez, and M. Curaba
Long-term spermatogonial survival in cryopreserved and xenografted immature human testicular tissue
Hum. Reprod., November 1, 2008; 23(11): 2402 - 2414.
[Abstract] [Full Text] [PDF]


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