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BOR - Papers in Press, published online ahead of print February 28, 2007.
Biol Reprod 2007, 10.1095/biolreprod.107.060079
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biolreprod.107.060079v1
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Submitted January 10, 2007
Returned for revision January 26, 2007
Accepted February 27, 2007

Testis


Polypyrimidine Tract Binding Protein 2 Stabilizes Phosphoglycerate Kinase 2 Messenger RNA in Murine Male Germ Cells by Binding to Its 3'UTR

Mingang Xu and Norman B. Hecht *

* To whom correspondence should be addressed. E-mail: nhecht{at}mail.med.upenn.edu.

Abstract
The mRNA encoding the testis-specific protein phosphoglycerate kinase (PGK2) is a long-lived mRNA transcribed in meiotic and post-meiotic male germ cells. Pgk2 mRNA is present in germ cells up to 2 weeks before its protein is detected. Using affinity chromatography with the 3’UTR of the Pgk2 mRNA, several proteins including the RNA-binding protein, polypyrimidine tract binding protein 2 (PTBP2), were identified in mouse testis extracts. Co-immunoprecipitations confirmed that PTBP2 binds to Pgk2 mRNA in the testis and RNA gel shifts demonstrated that PTBP2, but not PTBP1, binds to a specific region of the Pgk2 3'UTR. Recombinant PTBP2 increased the stability of reporter constructs containing the 3’UTR Pgk2 sequence element in both testis extracts and in transfected Hela cells. We propose that PTBP2 is a trans-acting factor that helps stabilize Pgk2 mRNA in male germ cells of the mouse.

Key words: Testis • Gametogenesis • Gene regulation • Spermatogenesis


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