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BOR - Papers in Press, published online ahead of print December 19, 2007.
Biol Reprod 2007, 10.1095/biolreprod.107.060871
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Submitted February 12, 2007
Returned for revision March 28, 2007
Accepted November 20, 2007

Testis


Adrenomedullin in the Rat Testis. I: Its Production, Actions on Testosterone Secretion, Regulation by Human Chorionic Gonadotropin, and Its Interaction with Endothelin 1 in the Leydig Cell

Yuen-Fan Chan , Wai-Sum O , and Fai Tang *

* To whom correspondence should be addressed. E-mail: ftang{at}hkucc.hku.hk.

Abstract
Based on the finding of gene expression of adrenomedullin (ADM) and its receptor components in the rat testis, a paracrine effect of ADM on testicular steroidogenesis has been suggested by our group. The present study demonstrates the gene expression of Adm and the effect of ADM on testosterone production in the Leydig cell. The regulation of ADM by hCG and its interaction with endothelin 1 (EDN1) in the rat Leydig cells are also observed. Primary culture of Leydig cells produced Adm mRNA and secreted 275 ± 19 pg of immunoreactive ADM per 106 cells in 24 hours. Beside, the Leydig cell was shown to co-express mRNAs encoding for the calcitonin receptor-like receptor (CALCRL) and receptor activity modifying protein (RAMP1, RAMP2 and RAMP3). These may account for the specific binding of ADM to the Leydig cells. Administration of ADM to Leydig cells resulted in an inhibition of hCG- and EDN1- stimulated testosterone production. Correlated with this, ADM reduced EDN1 production while its production was increased by EDN1. Furthermore, the production of ADM and the mRNA levels of Calcrl and Ramp2 were suppressed by hCG. Our results suggest that ADM has an autocrine effect on Leydig cell steroidogenesis, possibly by interacting with EDN1 and under the control of gonadotropin. We propose that there is an ADM/EDN1 local regulatory mechanism which may be important in modulating the control of testicular functions by gonadotropins.

Key words: Testis • Leydig cells • adrenomedullin • endothelin-1


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Y.-F. Chan, F. Tang, and W.-S. O
Adrenomedullin in the Rat Testis. II: Its Production, Actions on Inhibin Secretion, Regulation by Follicle-Stimulating Hormone, and Its Interaction with Endothelin 1 in the Sertoli Cell
Biol Reprod, April 1, 2008; 78(4): 780 - 785.
[Abstract] [Full Text] [PDF]


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