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BOR - Papers in Press, published online ahead of print May 2, 2007.
Biol Reprod 2007, 10.1095/biolreprod.107.061101
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Submitted February 23, 2007
Returned for revision March 12, 2007
Accepted April 25, 2007

Testis


Expression Analysis of the Mouse Multi-Copy X-Linked Gene Xlr-Related, Meiosis-Regulated (Xmr), Reveals That Xmr Encodes a Spermatid-Expressed Cytoplasmic Protein, SLX/XMR

Louise N. Reynard , James M.A. Turner , Julie Cocquet , Shantha K. Mahadevaiah , Aminata Toure , Christer Hoog , and Paul S. Burgoyne *

* To whom correspondence should be addressed. E-mail: pburgoy{at}nimr.mrc.ac.uk.

Abstract
The mouse multi-copy X-linked gene Xlr-related, meiosis-regulated (Xmr/Slx) has previously been described as encoding a testis-specific nuclear protein expressed during male meiotic prophase during which it becomes concentrated in the inactive X and Y chromatin domain; these conclusions were based on western and immunolocalization analysis using an antibody raised against a related lymphocyte protein, XLR. However, our recently published RNA in situ for Xmr revealed that transcripts are predominantly/exclusively post-meiotic and this is supported by accumulating microarray data. This led us to re-analyze the expression of Xmr, both at the RNA level by RTPCR and by RNA FISH, and also at the protein level using antibodies raised against XMR that do not recognize XLR. In agreement with our previous RNA in situ data, our further transcription analysis showed almost exclusive expression in spermatids, and western and immunostaining with the XMR antibodies showed that the protein is cytoplasmic and restricted to spermatids. Furthermore, the previously used XLR antibody was shown not to cross-react with XMR and it is suggested that the meiotically-expressed nuclear protein recognized by this antibody is another member of the complex Xlr superfamily. As a result of these findings, the gene previously known as Xmr is now officially know as Slx, Sycp3 like X-linked.

Key words: Testis • Gametogenesis • Meiosis • Spermatid • Spermatogenesis


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