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BOR - Papers in Press, published online ahead of print June 20, 2007.
Biol Reprod 2007, 10.1095/biolreprod.107.062273
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Submitted April 15, 2007
Returned for revision May 5, 2007
Accepted June 7, 2007

Ovary


Expression and Function of Fibroblast Growth Factor 10 and Its Receptor, Fibroblast Growth Factor Receptor 2B, in Bovine Follicles

J. Buratini, Jr. *, M. G.L. Pinto , A. C. Castilho , R. L. Amorim , I. C. Giometti , V. M. Portela , E. S. Nicola , and C. A. Price

* To whom correspondence should be addressed. E-mail: buratini{at}ibb.unesp.br.

Abstract
Some fibroblast growth factors (FGF) affect ovarian follicle cell growth and/or differentiation. Whereas many FGFs activate several FGF receptors, FGF7 and FGF10 primarily activate only one, FGFR2B. As FGF7 is produced by bovine theca cells and acts on granulosa cells, we tested the hypothesis that FGF10 may also play a role in folliculogenesis in cattle. Reverse transcription-polymerase chain reaction demonstrated the presence of FGF10 mRNA in oocytes and theca cells of antral follicles, and in preantral follicles. FGF10 protein was detected by immunohistochemistry in oocytes of preantral and antral follicles, and in granulosa and theca cells of antral follicles. FGF10 expression in theca cells changed during follicle development; mRNA abundance decreased with increasing follicular estradiol concentration in healthy follicles, and was lowest in highly atretic follicles. Culture of granulosa cells in serum-free medium demonstrated regulation of FGF10 receptor expression by FSH. Addition of FGF10 to cultured granulosa cells decreased estradiol but did not alter cell proliferation. These data support a role for FGF10 in signaling to granulosa cells from theca cells and/or the oocyte.

Key words: Follicle • Granulosa cells • Growth factors • Theca cells • Fibroblast growth factors


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